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ALK Fusion Gene Detection Kit

DiaCarta technologies deliver faster, more sensitive results for diagnostic and molecular genetic testing.

QFusion™ EML4-ALK and KIF5B-ALK Fusion Gene Detection Kit*

Tired of the FISH method? QFusion™ is the most comprehensive, rapid and reliable alternative to the laborious Fluorescence In Situ Hybridization (FISH) test. The QFusion™ single-tube qPCR assay is designed to accelerate your research in non-small cell lung cancer (NSCLC) by improving the quality and productivity of your EML4-ALK and KIF5B-ALK fusion gene variant analysis.

•  UNIQUE – The only commercial qPCR kit for EML4-ALK and KIF5B-ALK fusion variants
•  COMPREHENSIVE – Cover 95% EML4-ALK and 90% KIF5B-ALK variants in COSMIC database
•  EASY TO OPERATE – One-tube multiplex qPCR reactions and only 50 ng RNA as assay input
•  SPECIFIC & SENSITIVE – No cross reactivity with wild-type RNA & 50 fusion templates detected per reaction
•  RAPID – Total turnaround time approx. 1.5 hours
•  VERSATILE – Assay validated in wildly used real-time PCR machines

ALK fusion genes and cancer

EML4-ALK has been emerged as one of the most important driver oncogenes in lung cancer and the first targeted fusion onco-kinase to be identified in 4-6% of lung adenocarcinomas. Other non-EML4 fusion partner genes including KIF5B, TFG, and KLC1 have also been reported. Over the last few years, ALK inhibitors have shown significant benefits in the management of ALK-positive non-small cell lung cancer (NSCLC) compared to conventional chemotherapy.

Chromosomal translocations involving the anaplastic lymphoma kinase (ALK) gene as a 3’ partner have been found in ~5% of non-small cell lung cancer (NSCLC). These translocations fuse the kinase domain of ALK with other genes, producing a chimeric protein with oncogenic potential. Recently developed kinase inhibitors that target the kinase domain of ALK are currently used to treat ALK fusion positive NSCLC patients. Most frequent 5’ partners of these fusions are EML4 and the KIF5B genes.

Fusion gene variants detected

alk-fusion-detection-update

Product specifications

Fusion Variants:EML4-ALK: V1, V2, V3a/b, V5a/b, V6,V7, V8a/b KIF5B-ALK: (K15; A20), (K17; A20), (K24; A20)
Sample:FFPE tissues from non-small cell lung cancer (minimum 50 ng RNA)
Pack Size:5 samples; 20 samples
Instruments: Roche LC 96, LC 480 II, and Bio-Rad CFX384
Detection Channel:FAM, HEX
Turnaround Time:qPCR setup: ~0.5 hour; assay run time: ~1 hour
Stability:Stable for 12 months at -25℃ to -15℃

Ordering information

Product Name Sample SizeCatalog NumberIntended Use
QFusion™ EML4-ALK and KIF5B-ALK Fusion Gene Detection Kit 5 samplesDC-20-0005ECE/IVD
QFusion™ EML4-ALK and KIF5B-ALK Fusion Gene Detection Kit 20 samples DC-20-0020RResearch use only in North America
QFusion™ EML4-ALK and KIF5B-ALK Fusion Gene Detection Kit 5 samples DC-20-0005ECE/IVD
QFusion™ EML4-ALK and KIF5B-ALK Fusion Gene Detection Kit 20 samples DC-20-0020RResearch use only in North America
* For Research Use Only. Not for use in diagnostic procedures.