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Detects reliably below 0.1% mutant DNA by real-time PCR

QClamp® BRAF Mutation Test

The QClamp® BRAF Mutation Test in Codon 600 is a real-time PCR assay for the detection of somatic mutations in and near codon 600 in exon 15, in the BRAF serine/threonine protein kinase gene, using purified DNA extracted from FFPE (formalin fixed paraffin embedded), cells or tissue. The kit identifies the presence or absence of mutations in the targeted regions but does not specify the exact nature of the mutation. The codon specific detection kits are designed to detect any mutation at or near the stated codon site without specifying the exact nucleotide change.

HIGHLIGHTS

• Sample Ready: Suitable for FFPE, cell or tissue
• Ultra-Sensitive: Detects below 0.1% mutant DNA by real-time PCR
• Rapid: Streamlined workflow with less than 4 hours turnaround time
• Versatile: Works with most PCR machines
• Reproducible: Robust XNA-PCR technology minimizing variability

List of mutations and cosmic identities found in BRAF

Exon Amino Acid Change Nucleotide change Cosmic No.
15 V600>E c1799T>A 6239
V600>K c1798_1799GT>AA 6252
V600>D c1799_1800TG>AT 6253
V600>R c1798_1799GT>AG
V600>M  c1798G>A  6223

Product specifications

Specification QClamp® BRAF Codon Specific Mutation Test
Intended UseFor In Vitro Diagnostic Use (CE/IVD) or For Research Use
Sample TypeFFPE, Cells or Tissues
Pack Size10 Samples, 30 Samples and 60 Samples
Validated InstrumentsABI 7500, ABI 7500FAST, ABI 7900, ABI StepOnePlus, Qiagen Rotor-Gene Q, Roche LightCycler LC96, Roche LightCycler 480 II
Detection ChannelFAM; HEX
Turnaround TimeLess than 4 hours
StabilityStable for 12 months at -25 ℃ to -15 ℃

Ordering information

Product NameCatalog # (RUO)Catalog # (CE/IVD)
QClamp® BRAF Codon Specific Mutation Test in Codon 600 (10 Samples)DC-10-1039R DC-10-1039
QClamp® BRAF Codon Specific Mutation Test in Codon 600 (30 Samples)DC-10-0197RDC-10-0197
QClamp® BRAF Codon Specific Mutation Test in Codon 600 (60 Samples)DC-10-0169RDC-10-0169

Note: RUO (Research Use Only) products are not for use in diagnostic procedures. CE/IVD products are intended for use in diagnostic procedures.