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BRAF Mutation Test

QClamp® BRAF Mutation Test

The QClamp® BRAF Mutation Test in Codon 600 is a real-time PCR assay for the detection of somatic mutations in and near codon 600 in exon 15, in the BRAF serine/threonine protein kinase gene, using purified DNA extracted from FFPE (formalin fixed paraffin embedded), cells or tissue. The kit identifies the presence or absence of mutations in the targeted regions but does not specify the exact nature of the mutation. The codon specific detection kits are designed to detect any mutation at or near the stated codon site without specifying the exact nucleotide change.

QClamp® somatic mutation detection technology overview

QClamp® is a revolutionary new way to screen for somatic mutations that utilizes a sequence-specific clamp that suppresses PCR amplification of wild-type template DNA. QClamp® allows selective PCR amplification of only mutant templates, which allows the detection of mutant DNA in the presence of a large excess of wild-type templates from a variety of samples including fresh tissue, FFPE, ctDNA, whole blood and plasma.

Ordering information

QClamp™ BRAF Codon Specific Mutation Test in Codon 600
Related CodonsSample SizeCatalog NumberIntended Use
Exon 15 (Codon 600)10 SamplesDC-10-1039CE/IVD
10 SamplesDC-10-1039RRUO
30 SamplesDC-10-0197CE/IVD
30 SamplesDC-10-0197RRUO
60 SamplesDC-10-0169CE/IVD
60 SamplesDC-10-0169RRUO

QClamp® gene mutation detection kits features overview

Ultra-Sensitive

Detect ≤ 0.1% mutant DNA

Rapid

Turnaround time
~ 2 hours

Sample Ready

Suitable for liquid biopsy,
FFPE tissue and more

Versatile

Works with most
PCR machines

Cost Effective

Lower testing cost due to low cancellation rate and minimal amount needed of DNA per assay

List of mutations and cosmic identities found in BRAF

Exon Amino Acid Change Nucleotide change Cosmic No.
15 V600>E c1799T>A 6239
V600>K c1798_1799GT>AA 6252
V600>D c1799_1800TG>AT 6253
V600>R c1798_1799GT>AG
V600>M  c1798G>A  6223