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Detects reliably below 0.1% mutant DNA by real-time PCR

QClamp® c-KIT Mutation Test

The QClamp® c-KIT Mutation Test in Codon 816 is a real-time qualitative PCR assay for the detection of somatic mutations in Codon 861 of Exon 17 in the c-KIT proto-oncogene in genomic DNA from human cells or tissues. The kit identifies the presence or absence of mutations in the targeted regions but does not specify the exact nature of the mutation, it is designed to detect any mutation at or near the stated codon site without specifying the exact nucleotide change. The wild-type allele of the c-KIT gene encodes an aspartic acid residue (D) at codon 816 (nucleotide position c.2446-2448 GAC).

HIGHLIGHTS

• Sample Ready: Suitable for FFPE, cell or tissue
• Ultra-Sensitive: Detects below 0.1% mutant DNA by real-time PCR
• Rapid: Streamlined workflow with less than 4 hours turnaround time
• Versatile: Works with most PCR machines
• Reproducible: Robust XNA-PCR technology minimizing variability

List of mutations and cosmic identities found in c-KIT

Exon  Amino Acid Change  Nucleotide change Cosmic No.
17 D816H  c.2446G>C or c. 2446_2448CAG>CAT 1311 or 1600404
D816E  c.2448C>G 19285
D816V  c.2447A>T 1314
D816G  c.2447A>G 12711
D816A  c.2447A>C 24675
D816Y  c.2446G>T 1310
D816F  c.2446_2447GA>TT 1312
D816I  c. 2446_2447GA>AT 21979

Product specifications

Specification QClamp® c-KIT Codon Specific Mutation Test
Intended UseFor Research Use
Sample TypeFFPE, Cells or Tissues
Pack Size10 Samples, 30 Samples and 60 Samples
Validated InstrumentsBioRad CFX384, Roche LC96, Roche LC480
Detection ChannelFAM; HEX
Turnaround TimeLess than 4 hours
StabilityStable for 12 months at -25 ℃ to -15 ℃

Ordering information

Product NameCatalog # (RUO)Catalog # (CE/IVD)
QClamp® c-KIT Codon Specific Mutation Test in Codon 816 (10 Samples) DC-10-1059R N/A
QClamp® c-KIT Codon Specific Mutation Test in Codon 816 (30 Samples) DC-10-0501R N/A
QClamp® c-KIT Codon Specific Mutation Test in Codon 816 (60 Samples) DC-10-0504R N/A

Note: RUO (Research Use Only) products are not for use in diagnostic procedures. CE/IVD products are intended for use in diagnostic procedures.