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c-KIT Mutation Test

QClamp® c-KIT Mutation Test

The QClamp® c-KIT Mutation Test in Codon 816 is a real-time qualitative PCR assay for the detection of somatic mutations in Codon 861 of Exon 17 in the c-KIT proto-oncogene in genomic DNA from human cells or tissues. The kit identifies the presence or absence of mutations in the targeted regions but does not specify the exact nature of the mutation, it is designed to detect any mutation at or near the stated codon site without specifying the exact nucleotide change. The wild-type allele of the c-KIT gene encodes an aspartic acid residue (D) at codon 816 (nucleotide position c.2446-2448 GAC

Available kits

QClamp™ c-KIT Codon Specific Mutation Test in Codon 816
Related CodonsSample SizeCatalog NumberIntended Use
Exon 17 (Codon 816)10 SamplesDC-10-1059RRUO
30 SamplesDC-10-0501RRUO
60 SamplesDC-10-0504RRUO

QClamp® somatic mutation detection technology overview

QClamp® is a revolutionary new way to screen for somatic mutations that utilizes a sequence-specific clamp that suppresses PCR amplification of wild-type template DNA. QClamp® allows selective PCR amplification of only mutant templates, which allows the detection of mutant DNA in the presence of a large excess of wild-type templates from a variety of samples including fresh tissue, FFPE, ctDNA, whole blood and plasma.

QClamp® gene mutation detection kits features overview

Ultra-Sensitive

Detect ≤ 0.1% mutant DNA

Rapid

Turnaround time
~ 2 hours

Sample Ready

Suitable for liquid biopsy,
FFPE tissue and more

Versatile

Works with most
PCR machines

Cost Effective

Lower testing cost due to low cancellation rate and minimal amount needed of DNA per assay

List of mutations and cosmic identities found in c-KIT

Exon  Amino Acid Change  Nucleotide change Cosmic No.
17 D816H  c.2446G>C or c. 2446_2448CAG>CAT 1311 or 1600404
D816E  c.2448C>G 19285
D816V  c.2447A>T 1314
D816G  c.2447A>G 12711
D816A  c.2447A>C 24675
D816Y  c.2446G>T 1310
D816F  c.2446_2447GA>TT 1312
D816I  c. 2446_2447GA>AT 21979