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KRAS Mutation Test

QClamp® KRAS Mutation Test

The QClamp® KRAS  Mutation Test in Codons 12, 13, 59, 61, 117, & 146 is a real-time qualitative PCR assay for the detection of somatic mutations in and near Codons 12 and 13 in Exon 2, Codons 59 and 61 in Exon 3, and Codons 117 and 146 in Exon 4 in the human KRAS gene, using purified DNA extracted from FFPE (formalin fixed paraffin embedded), cells or tissue. The kit identifies the presence or absence of mutations in the targeted regions but does not specify the exact nature of the mutation. The codon specific detection kits are designed to detect any mutation at or near the stated codon site without specifying the exact nucleotide change.

QClamp® somatic mutation detection technology overview

QClamp® is a revolutionary new way to screen for somatic mutations that utilizes a sequence-specific clamp that suppresses PCR amplification of wild-type template DNA. QClamp® allows selective PCR amplification of only mutant templates, which allows the detection of mutant DNA in the presence of a large excess of wild-type templates from a variety of samples including fresh tissue, FFPE, ctDNA, whole blood and plasma.

Ordering information

QClamp™ KRAS Codon Specific Mutation Test in Codons 12, 13, 59, 61, 117 & 146
Related CodonsSample SizeCatalog NumberIntended Use
Exon 2 (Codon 12, Codon 13)
Exon 3 (Codon 59, Codon 61)
Exon 4 (Codon 117, Codon 146)
10 SamplesDC-10-2010CE/IVD
10 SamplesDC-10-2010RRUO
30 SamplesDC-10-3010CE/IVD
30 SamplesDC-10-3010RRUO
60 SamplesDC-10-4010CE/IVD
60 SamplesDC-10-4010RRUO
QClamp™ KRAS Codon Specific Mutation Test in Codons 12, 13, 61, 117 & 146
Related CodonsSample SizeCatalog NumberIntended Use
Exon 2 (Codon 12, Codon 13) Exon 3 (Codon 61) Exon 4 (Codon 117, Codon 146)10 SamplesDC-10-1033CE/IVD
10 SamplesDC-10-1033RRUO
30 SamplesDC-10-0170CE/IVD
30 SamplesDC-10-0170RRUO
60 SamplesDC-10-0171CE/IVD
60 SamplesDC-10-0171RRUO
QClamp™ KRAS Codon Specific Mutation Test in Codons 12, 13 & 61
Related CodonsSample SizeCatalog NumberIntended Use
Exon 2 (Codon 12, Codon 13) Exon 3 (Codon 61) 10 SamplesDC-10-1066CE/IVD
10 SamplesDC-10-1066RRUO
30 SamplesDC-10-0036CE/IVD
30 SamplesDC-10-0036RRUO
60 SamplesDC-10-0039CE/IVD
60 SamplesDC-10-0039RRUO

QClamp® gene mutation detection kits features overview

Ultra-Sensitive

Detect ≤ 0.1% mutant DNA

Rapid

Turnaround time
~ 2 hours

Sample Ready

Suitable for liquid biopsy,
FFPE tissue and more

Versatile

Works with most
PCR machines

Cost Effective

Lower testing cost due to low cancellation rate and minimal amount needed of DNA per assay

List of mutations and cosmic identities found in KRAS

Exon Amino Acid Change Nucleotide change Cosmic No.
2 G12>A c.35G>C 522
G12>R  c.34G>C 518
G12>D  c.35G>A 521
G12>C  c.34G>T 516
 G12>S  c.34G>A 517
G12>V  c.35G>T 520
G13>D c.38G>A 532
G13>C  c.37G>T 527
G13>R  c.37G>C 529
3 A59>T  c.175G>A  546
Q61>H  c.183A>C & c.183A>T 554/555
Q61>K  c.181C>A 549
Q61>L  c.182A>T 553
Q61>R  c.182A>G 552
4 K117>E c.349A>G  1360831
K117>N  c.351A>C & c.351A>T  19940/28519
K117>R 350A>G
A146>A  c.438A>G 1360826
A146>G  c.437C>G  1360829
A146>P  436G>C 19905
A146>T  436G>A 19404
A146>V  437C>T>C  19900