Order Now 1-800-246-8878

Detects reliably below 0.1% mutant DNA by real-time PCR

QClamp® NRAS Mutation Test

The QClamp® NRAS Mutation Test in Codons 12, 13, 59, 61, 117, & 146 is a real-time qualitative PCR assay for the detection of somatic mutations in and near NRAS Codon 12 and 13 in Exon 2, Codon 59 and 61 in Exon 3, and Codon 146 in Exon 4 in the human NRAS gene, using purified DNA extracted from FFPE (formalin fixed paraffin embedded), cells or tissue. The kit identifies the presence or absence of mutations in the targeted regions but does not specify the exact nature of the mutation.

HIGHLIGHTS

• Sample Ready: Suitable for FFPE, cell or tissue
• Ultra-Sensitive: Detects below 0.1% mutant DNA by real-time PCR
• Rapid: Streamlined workflow with less than 4 hours turnaround time
• Versatile: Works with most PCR machines
• Reproducible: Robust XNA-PCR technology minimizing variability

List of mutations and cosmic identities found in NRAS

Exon Amino Acid Change Nucleotide change Cosmic No.
2 G12>D c.35G>A 564
G12>C c.34G>T 562
G12>S c.34G>A 563
G13>R c.37G>C 569
G13>V c.38G>T 574
3 A59>  c.175G>A 578
A59>D  c.176C>A 253327
G60>R  c.178G>C 46507
G60>E  c.179G>A 28673
Q61>E  c.181C>G 581
Q61>H  c.183A>C & c.183A>T 586/585
Q61>L  c.182A>T, c.182_183AA>TG, & c.181_182CA>TT 583/30646/
12725  c.182A>T 553
Q61>K  c.181C>A & c.181_183CAA>AAG 580/53223
Q61>P  c.182A>C 582
Q61>R  c.182A>G, c.181_182CA>AG, & c.182_183AA>GG 584/579/ 33696
Q61>Q  c.183A>G 587
4 A146>T c.436G>A 19404
K117>N  c.231A>G N/A

Product specifications

Specification QClamp® NRAS Codon Specific Mutation Test
Intended UseFor In Vitro Diagnostic Use (CE/IVD) or For Research Use
Sample TypeFFPE, Cells or Tissues
Pack Size10 Samples, 30 Samples and 60 Samples
Validated InstrumentsABI 790, QIAGEN Rotor-Gene Q
Detection ChannelFAM; HEX
Turnaround TimeLess than 4 hours
StabilityStable for 12 months at -25 ℃ to -15 ℃

Ordering information

Product NameCatalog # (RUO)Catalog # (CE/IVD)
QClamp® NRAS Codon Specific Mutation Test in Codons 12, 13, 59, 61, 117 & 146 (10 Samples)DC-10-2020R DC-10-2020
QClamp® NRAS Codon Specific Mutation Test in Codons 12, 13, 59, 61, 117 & 146 (30 Samples)DC-10-3020R DC-10-3020
QClamp® NRAS Codon Specific Mutation Test in Codons 12, 13, 59, 61, 117 & 146 (60 Samples)DC-10-4020R DC-10-4020

Note: RUO (Research Use Only) products are not for use in diagnostic procedures. CE/IVD products are intended for use in diagnostic procedures.