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NRAS Mutation Test

QClamp® NRAS Mutation Test

The QClamp® NRAS Mutation Test in Codons 12, 13, 59, 61, 117, & 146 is a real-time qualitative PCR assay for the detection of somatic mutations in and near NRAS Codon 12 and 13 in Exon 2, Codon 59 and 61 in Exon 3, and Codon 146 in Exon 4 in the human NRAS gene, using purified DNA extracted from FFPE (formalin fixed paraffin embedded), cells or tissue. The kit identifies the presence or absence of mutations in the targeted regions but does not specify the exact nature of the mutation.

QClamp® somatic mutation detection technology overview

QClamp® is a revolutionary new way to screen for somatic mutations that utilizes a sequence-specific clamp that suppresses PCR amplification of wild-type template DNA. QClamp® allows selective PCR amplification of only mutant templates, which allows the detection of mutant DNA in the presence of a large excess of wild-type templates from a variety of samples including fresh tissue, FFPE, ctDNA, whole blood and plasma.

Ordering information

QClamp™ NRAS Codon Specific Mutation Test in Codons 12, 13, 59 ,61, 117 & 146
Related CodonsSample SizeCatalog NumberIntended Use
Exon 2 (Codon 12, Codon 13) Exon 3 (Codon 59, Codon 61) Exon 4 (Codon 117, Codon 146)10 SamplesDC-10-2020CE/IVD
10 SamplesDC-10-2020RRUO
30 SamplesDC-10-3020CE/IVD
30 SamplesDC-10-3020RRUO
60 SamplesDC-10-4020CE/IVD
60 SamplesDC-10-4020RRUO
QClamp™ NRAS Codon Specific Mutation Test in Codons 12, 13, 61 & 146
Related CodonsSample SizeCatalog NumberIntended Use
Exon 2 (Codon 12, Codon 13) Exon 3 (Codon 61) Exon 4 (Codon 146)10 SamplesDC-10-1034CE/IVD
10 SamplesDC-10-1034RRUO
30 SamplesDC-10-0172CE/IVD
30 SamplesDC-10-0172RRUO
60 SamplesDC-10-0173CE/IVD
60 SamplesDC-10-017RRUO
QClamp™ NRAS Codon Specific Mutation Test in Codons 12, 13 & 61
Related CodonsSample SizeCatalog NumberIntended Use
Exon 2 (Codon 12, Codon 13) Exon 3 (Codon 61) 10 SamplesDC-10-1043CE/IVD
10 SamplesDC-10-1043RRUO
30 SamplesDC-10-0048CE/IVD
30 SamplesDC-10-0048RRUO
60 SamplesDC-10-0051CE/IVD
60 SamplesDC-10-0051RRUO

QClamp® gene mutation detection kits features overview

Ultra-Sensitive

Detect ≤ 0.1% mutant DNA

Rapid

Turnaround time
~ 2 hours

Sample Ready

Suitable for liquid biopsy,
FFPE tissue and more

Versatile

Works with most
PCR machines

Cost Effective

Lower testing cost due to low cancellation rate and minimal amount needed of DNA per assay

List of mutations and cosmic identities found in NRAS

Exon Amino Acid Change Nucleotide change Cosmic No.
2 G12>D c.35G>A 564
G12>C c.34G>T 562
G12>S c.34G>A 563
G13>R c.37G>C 569
G13>V c.38G>T 574
3 A59>  c.175G>A 578
A59>D  c.176C>A 253327
G60>R  c.178G>C 46507
G60>E  c.179G>A 28673
Q61>E  c.181C>G 581
Q61>H  c.183A>C & c.183A>T 586/585
Q61>L  c.182A>T, c.182_183AA>TG, & c.181_182CA>TT 583/30646/
12725  c.182A>T 553
Q61>K  c.181C>A & c.181_183CAA>AAG 580/53223
Q61>P  c.182A>C 582
Q61>R  c.182A>G, c.181_182CA>AG, & c.182_183AA>GG 584/579/ 33696
Q61>Q  c.183A>G 587
4 A146>T c.436G>A 19404
K117>N  c.231A>G N/A