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Detects reliably below 0.1% mutant DNA by real-time PCR

QClamp® PIK3CA Mutation Test

The QClamp® PIK3CA Mutation Test in Codons 542, 545, & 1047 is a real-time qualitative PCR assay for the detection of somatic mutations in and near PIK3CA Codons 542 and 545 in Exon 9 and Codon 1047 in Exon 20 in the human PIK3CA gene, using purified DNA extracted from FFPE (formalin fixed paraffin embedded), cells or tissue. The kit identifies the presence or absence of mutations in the targeted regions but does not specify the exact nature of the mutation. The codon specific detection kits are designed to detect any mutation at or near the stated codon site without specifying the exact nucleotide change.

HIGHLIGHTS

• Sample Ready: Suitable for FFPE, cell or tissue
• Ultra-Sensitive: Detects below 0.1% mutant DNA by real-time PCR
• Rapid: Streamlined workflow with less than 4 hours turnaround time
• Versatile: Works with most PCR machines
• Reproducible: Robust XNA-PCR technology minimizing variability

List of mutations and cosmic identities found in PIK3CA

Exon Amino Acid Change Nucleotide change Cosmic No.
9 E542>K c.1624G>A 760
E542>G c.1625A>G 761
E542>V c.1625A>T 762
E545>K c.1633G>A 763
E545>G c.1634A>G 764
E545>D c.1635G>T 765
20 H1047>Y c.3139C>T 774
H1047>L c.3140A>T 776
H1047>R c.3140A>G 775

Product specifications

Specification QClamp® PIK3CA Codon Specific Mutation Test
Intended UseFor In Vitro Diagnostic Use (CE/IVD) or For Research Use
Sample TypeFFPE, Cells or Tissues
Pack Size10 Samples, 30 Samples and 60 Samples
Validated InstrumentsABI 7500, ABI 7500FAST, ABI 7900, ABI StepOnePlus, Qiagen Rotor-Gene Q, Roche LightCycler LC96, Roche LightCycler 480 II
Detection ChannelFAM; HEX
Turnaround TimeLess than 4 hours
StabilityStable for 12 months at -25 ℃ to -15 ℃

Ordering information

Product NameCatalog # (RUO)Catalog # (CE/IVD)
QClamp® PIK3CA Codon Specific Mutation Test in Codons 542, 545 & 1047 (10 Samples)DC-10-0153RDC-10-0153
QClamp® PIK3CA Codon Specific Mutation Test in Codons 542, 545 & 1047 (30 Samples)DC-10-1072R DC-10-1072
QClamp® PIK3CA Codon Specific Mutation Test in Codons 542, 545 & 1047 (60 Samples)DC-10-1075R DC-10-1075

Note: RUO (Research Use Only) products are not for use in diagnostic procedures. CE/IVD products are intended for use in diagnostic procedures.