QuantiDNA™

cfDNA Extraction Kits

Manual Kit and Automated Kit

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Research Use Product

DiaCarta offers high-quality and cost-effective cfDNA extraction kits for both manual and automated extraction from 1 to 10 ml of plasma.

The purification process takes about 50 minutes with two-step extraction steps using either magnetic beads alone (automated) or magnetic beads plus spin columns.

High-Quality & Cost-Effective

The purified cfDNA using QuantiDNA™ cfDNA Extraction Kits has a similar yield and integrity to Qiagen’s QIAamp MinElute ccfDNA Midi Kit and is inhibitor-free for downstream analysis.

QuantiDNA™ Manual cfDNA Extraction Kits

  • Catalog Number: DC-12-0002R
  • Pack Size: 50 samples (for 4mL plasma/sample)

QuantiDNA™ Automated cfDNA Extraction Kits

  • Catalog Number: DC-12-0003R
  • Pack Size: 50 samples (for 4mL plasma/sample)

Try a FREE 5-sample cfDNA manual extraction kit today at your shipping expense!

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The Background of cfDNA Extraction

In recent years, liquid biopsy has become a powerful alternative to tissue biopsy. Although biopsy is the gold standard to diagnose diseases (cell biology and molecular biology), biopsy is not always easy or possible to get. In these cases, liquid biopsy is useful for disease diagnosis, progression monitoring, and therapy responses. In these applications, cfDNA (cell-free DNA) and a small fraction of cfDNA-ctDNA (circulating tumor DNA) are often used as biomarkers in terms of cfDNA quantification, ctDNA mutation or methylation detection (qPCR, digital droplet PCR, or NGS).

For these applications, cfDNA is often required to be extracted from the blood samples before downstream analysis. However, the cfDNA level, especially in healthy individuals, is often very low. Extraction of cfDNA often leads to a big fraction of cfDNA loss, jeopardizing the accuracy and sensitivity of the clinical testing result. High-quality and high-yield cfDNA extraction become critical.

Manual cfDNA Extraction Workflow

Here is the workflow for manual cfDNA extraction. After plasma isolation, the two-step extraction by magnetic beads and column is applied to ensure the cfDNA extracted is pure and free of inhibitors on downstream PCR reactions.

Plasma Isolation

20 mins

Proteinase K Treatment

15 mins

First Step Extraction

5 mins

Second Step Extraction

20 mins

Automated cfDNA Extraction Workflow

Unlike the manual cfDNA extraction kits, magnetic beads are also used for the second step of cfDNA extraction. The first- and second-step extractions are conducted on the automated extraction instrument MGISP-NE384.

Plasma Isolation

20 mins

Proteinase K Treatment

15 mins

First Step Extraction

23 mins

Second Step Extraction

41 mins

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