OptiSeq™ Pan-Cancer Panel Service (65-Gene)

Sample to report within 10 business days

DiaDiaCarta offers a sample-to-report OptiSeq™ Targeted Sequencing cancer service at its state-of-the-art facility. The liquid biopsy/FFPE sample prep workflow provides clients with fast turnaround time and accurate VAF detection process. Variants are classified based on guidelines with a focus on actionable content from drug labels and clinical trials. Information is presented in a final report. The whole process from samples (blood/FFPE) to report is completed within 10 business days in our laboratory.

OptiSeq™ Pan-Cancer Panel is designed to target 2,900 commonly observed mutational positions (hotspots) from 65 oncogenes and tumor suppressor genes. This panel contains a single pool of 601 primer pairs and the average amplicon length is 145 bp – perfectly suitable for ctDNA and FFDNA sequencing.

OptiSeq™ Pan-Cancer Gene Panel (65 Genes)

ABL1AKT1ALKAPCATMBRAFBRCA1
BRCA2CDH1CDKN2ACSF1RCTNNB1DDR2DNMT3A
EGFRERBB2ERBB3ERBB4EZH2FBXW7FGFR1
FGFR2FGFR3FLT3FOXL2GNA11GNAQGNAS
HNF1AHRASIDH1IDH2JAK2JAK3KDR
KITKRASMAP2K1METMLH1MPLMSH6
MTORNF1NF2NOTCH1NPM1NRASPDGFRA
PIK3CAPIK3R1PTCH1PTENPTPN11RB1RET
SMAD4SMARCB1SMOSRCSTK11TERTTP53
TSC1VHL

Advantages of OptiSeq™ Pan-Cancer Panel

samples tested

Liquid biopsy (blood/ccfDNA) and FFPE samples (tissue/DNA)  

highly sensitive

Highly sensitive NGS test allows as low as 1% variant allele frequency (VAF) detection

Fast turnaround time

Sample receipt to clinical report within 10 days. No deep sequencing  

Extremely uniform

100% observed uniformity at ≥ 0.2X mean depth and 97% observed uniformity at ≥ 0.5X

Low dna input needed

10ng for detection of somatic mutations (SNP); 100pg for germline mutations

Report to Clinicians

Comprehensive clinically significant variants, FDA-approved therapies and clinical trials

OptiSeq™ Pan-Cancer Panel Service Sample Report

The research NGS report also includes information regarding variant details, FDA-approved therapies for this indication, therapies associated with resistance, clinical trials, genes tested, methods and limitations, and selected citations.

Streamlined Workflow for OptiSeq™ Pan-Cancer Panel

Step 1: Sample prep & QC

Step 2: Target enrichment

Step 3: Library Preparation & QC & Template

Step 4: Sequencing Run

Step 5: Data Analysis

Step 6: Reporting

OptiSeq™ Pan-Cancer Panel Specifications

Sample Type

Liquid biopsy (blood/cfDNA) and FFPE samples (tissue/DNA)

Species

Human

Recommended DNA Input (Amount)

10ng for detection of somatic mutations (SNP); 100pg for germline mutations

Sequencing Platform

Illumina Sequencers (MiniSeq, MiSeq, NextSeq, HiSeq)

Enrichment Method

Multiplex PCR

Number of Primer Pairs

601

Number of Primer Pools

1

Number of Target Genes

65

Target Region Size (bp)

55199

Amplicon Size

Average 146bp (from 125-175bp)

Sample Multiplexing (at ~2000x mean coverage)

Miseq 2×150 Read Length: ~25 samples
NextSeq Series Mid Output 2×150 Read Length: ~200
NextSeq Series High Output @ 2×150 Read Length: ~600

Coverage Uniformity (at ~ ≥ 0.2x Mean Coverage)

> 95%

On-target Reads % (% reads aligned to target regions out of total aligned reads)

> 95%

Turnaround Time

Sample receipt to report within 10 days

Resources

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