DiaCarta™ Plex Immunoassays
Antibody and Magnetic Bead-Based Immunoassays for Comprehensive Multiplex Detection
DiaCarta™ Plex Immunoassays overcome the limitations of single-analyte ELISA by using antigen-antibody interactions to measure multiple targets or analytes using beads format in a single well. They are widely used in life science research and pharmaceutical drug discovery.
The applications of the assays include, but are not limited to
Quantification of immune responses
Identification and tracking of cancer biomarkers
Characterization of cellular products for cell and gene therapy
Discovery and study of a set of biomarkers contributing to a specific disease or indicating responses to medical treatment
Basic research in a variety of areas to understand the mechanism and processes
DiaCarta™ Plex assays are antibody and magnetic bead-based immunoassays
Each assay can analyze up to 21 protein targets simultaneously in a single well using only 15 μL of serum, plasma, other body fluids, or cell culture supernatants. The magnetic beads used in our assays are colored with a unique combination of two fluorescence dyes at different concentrations which can be analyzed by Luminex instruments or by any standard flow cytometers, including the high-end Cytek Aurora.
Researchers can use DiaCarta™ Plex kits in a fixed design panel or can cherry-pick the panels of their own interest.
Figure 1: Target IL-8 standard curves plotted based on Luminex MAGPIX (left top and bottom panels) and on Cytek Aurora (right top and bottom panels). Both platforms show comparable results.
Figure 2: The 18 cytokine levels in the plasma of 6 different cancer patients detected by Luminex 200.
How does DiaCarta™ Plex work?
The DiaCarta Plex Immunoassay is based on the principles of sandwich ELISA. In the assay, magnetic beads, conjugated with target-specific antibodies, bind the proteins of interest such as a cytokine during the initial 60-minute incubation step. The following steps consist of a 30-minute hybridization with biotinylated antibody against a secondary epitope of the protein and a subsequent 10-minute streptavidin phycoerythrin (SAPE) incubation step. The fluorescent intensity of SAPE on the beads is measured with a standard Luminex instrument or a flow cytometer equipped with a 488nm laser. The amount of the targeted protein in a sample can be quantified using a standard curve.
Assay Specifications
Sample Types
Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate
Sensitivity (LOD)
Majority of the assays < 10 pg/mL
Quantitation Range
LLOQ: Majority of the assays < 20 pg/mL; ULOQ: Majority of the assays > 5,000 pg/mL
Standard Dose Recovery
70-130%
Precision
Intra-assay CV: < 10%; Inter-assay CV: < 20%
Cross-reactivity of Analytes in a Panel or Group
Negligible
Sample Volume
15 μL
DiaCarta Offerings
Human Cytokine 21-Plex Panel
Includes: CCL2, CCL3, CCL4, CCL5, CSF2, CXCL10, IL1A, IL1B, IL4, IL6, IL8, IL10, IL12B, IL13, IL17A, IFNA1, IFNG, ICAM1, SELE, SELP, and TNF.
- Pack size: 96 test
Human Inflammation 16-Plex Panel
Includes: IL1A, IL1B, IL6, IL8, IL10, IL12B, IL13, IL17A, IL27, IL31, IL33, CXCL10, CCL2, CCL3, TNF, and IFNG.
- Pack size: 96 test
Mouse Cytokine 21-Plex Panel
Includes: CCL2, CCL3, CCL4, CCL5, CCL7, CCL11, CSF2, CXCL1, CXCL10, IFNG, IL1B, IL2, IL4, IL6, IL12B, IL13, IL9, IL10, IL17A, IL22, and TNF.
- Pack size: 96 test
Mouse Inflammation 16-Plex Panel
Includes: IFNG, IL1A, IL1B, IL6, IL9, IL10, IL12B, IL13, IL15, CXCL1, CXCL10, CCL2, CCL3, CCL4, CCL5, and TNF.
- Pack size: 96 test
Make a Customized Panel?
Contact DiaCarta at order@diacarta.com to place the order
In your email, please specify your targets/ analytes and your matrix.
All customized panels require 2 to 4 weeks for production. Researchers are required to pay 50% of the total cost upfront to initiate panel production, with the remaining balance due upon delivery.