QClamp® JAK2 Mutation Detection Tests
Improved Sensitivity for Single Gene Mutation Detection
The QClamp® JAK2 Mutation Detection Test for plasma sample aids in the identification of cancer patients eligible for treatment and in monitoring response to therapy, which can lead to improved outcomes in cancer patients. The QClamp® JAK2 Mutation Detection Test is an in vitro diagnostic real-time quantitative PCR assay for the detection of somatic mutations in and near JAK2 codon 617 in Exon 14 in the JAK2 tyrosine kinase gene, using purified DNA extracted from plasma.
Powered by XNA technology, the QClamp® JAK2 Mutation Detection Test has achieved a much higher analytical sensitivity compared to other commercial qPCR kits and other cancer gene mutation detection methods. QClamp® JAK2 Mutation Detection Test is able to detect reliably 0.1% to 0.5% mutant DNA out of wild-type DNA for targeted mutations, providing lower detection limit compared to similar assays available in the market due to robust enrichment of mutant sequences while suppressing amplification of wild-type sequences.
Product Catalog
QClamp® JAK2 Mutation Detection Test – CE Version: DC-10-0166
QClamp® JAK2 Mutation Detection Test – Research-Use Version: DC-10-0166R
Pack Size: 30 Samples
Detected Codon
Codon 617
Service Offering
We provide research service for QClamp® JAK2 Mutation Detection Test.
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Advantages of QClamp® JAK2 Mutation Detection Test
ULTRA-SENSITIVE
Reliably detects 0.1% to 0.5% VAF mutant DNA out of wild-type DNA for targeted mutations
SAMPLE READY
Suitable for plasma samples
LOW INPUT DNA
Minimum 5ng input DNA per reaction. Less than 2 tubes of blood (10mL each) needed for cfDNA
COMPREHENSIVE COVERAGE
Covering all relevant somatic mutations of JAK2 oncogene
FAST RESULTS
Less than 4 hours of assay run time
GREAT VERSATILITY
Validated on the most common qPCR machines with minimized variability
JAK2 Mutation and Cancer
JAK2 Introduction
Janus kinase 2 (JAK2) is a non-receptor tyrosine kinase that associates with the cytoplasmic domains of multiple cytokine receptors to activate downstream targets, including STATs and JAK2 itself.
JAK2 Mutations
V617F mutation in JAK2 leads to constitutive tyrosine phosphorylation activity and constitutive activation of STAT5. This mutation is common in the following cases: >90% of patients with polycythemia vera, 50% of patients with primary myelofibrosis and 60% of patients with essential thromobocytopenia.
Supporting Data for QClamp® JAK2 Mutation Detection Test
QClamp® JAK2 Mutation Detection Test amplification plot from ABI QuantStudio 5
Amplification plot showing triplicates of positive controls (mutant template and wild-type template mix) and negative controls (wild-type templates only) for JAK2 TaqMan amplification using ABI QuantStudio 5. Reference gene ACTB amplification from the positive and negative controls (total 6) is shown as well.
QClamp® JAK2 Mutation Detection Test amplification plot from Bio-Rad CFX384
Amplification plot showing triplicates of positive controls (mutant template and wild-type template mix) and negative controls (wild-type templates only) for JAK2 TaqMan amplification using Bio-Rad CFX384. Reference gene ACTB amplification from the positive and negative controls (total 6) is shown as well.
QClamp® JAK2 Mutation Detection Test amplification plot from Roche LightCycler® 480 (FAM)
Amplification plot showing triplicates of positive controls (mutant template and wild-type template mix) and negative controls (wild-type templates only) for JAK2 TaqMan amplification using Roche LightCycler® 480.
Streamlined Workflow for QClamp® Gene Mutation Detection Tests
Step 1: DNA Isolation & Quantification
Extract DNA from FFPE or plasma using a commercial DNA extraction kit and measure the concentration using fluorometric analysis
Step 2: set up qpcr
Mix the assay reagents, load into PCR plate, add controls and extracted DNA ~ 30-60 minutes
Step 3: Amplification parameters
Enter amplification parameters on
qPCR instrument, load PCR plate
and start the run ~ 2.5 hours
Step 4: Data analysis
Determine the presence or absence
of mutations according to the Cq
value cutoffs ~ 15 minutes
Resources
Catalog Number
CE Catalog #: DC-10-0166
Research-use-only (RUO) catalog #: DC-10-0166R
Pack Size
30 samples
Detected Codons
Codon 617
Sample Type
Plasma
Input DNA
5-10ng/Reaction
Validated Instruments
Roche LightCycler® 480, Bio-Rad CFX384 and ABI QuantStudio 5
Detection Channel
FAM; HEX
Detection Chemistry
TaqMan
Turnaround Time
Less than 4 hours
Stability
Stable for 12 months at -25 ℃ to -15 ℃
Most frequent JAK2 mutations detected by QClamp® JAK2 Mutation Detection Test
| Exon | Amino Acid Change | Nucleotide Change | Cosmic No. |
|---|---|---|---|
| 14 | V617>F | 1849G>T | 12600 |
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Ordering Information
For products that are in stock, DiaCarta will arrange shipment in 1-3 days. For products that are on backorder, DiaCarta will arrange shipment in 3-5 weeks.
Intended Use: QClamp® JAK2 Mutation Detection Test is CE/IVD-certified. Outside
Shipping Condition: QClamp® JAK2 Mutation Detection Test will be shipped with dry ice. For domestic shipment, DiaCarta provides overnight delivery through FedEx Domestic Overnight Shipping Service. For international shipment, DiaCarta provides 3-7 days in transit through FedEx International Priority Shipping Service. Please contact DiaCarta if you prefer to use your own shipping carrier.