EML4-ALK has been emerged as one of the most important driver oncogenes in lung cancer and the first targeted fusion
QFusion™ ALK fusion gene detection kits provide an easy-to-use multiplex RT-qPCR assay that simultaneously detects the most prevalent EML4-ALK and KIF5B gene fusion breakpoints. This highly specific and sensitive assay requires a minimal amount of RNA, and it is a rapid and reliable alternative to the laborious FISH test.
QFusion™ EML4-ALK and KIF5B-ALK Fusion Gene Detection Test is CE/IVD-certified.
ALK Fusion Gene Variants Detected
Amplification Curve Generated by QFusion™ for FFPE Reference Materials
EML4-ALK fusion gene variant 1 could be successfully detected from the ALK fusion-positive FFPE reference material (RM) (blue line) but not the negative RM (green line). The internal control (IC) assay similarly amplified the positive and negative FFPE RMs (black and red lines), indicating that the total RNA input (50 ng) extracted from these RMs was about the same.
Advantages of QFusion™ ALK Fusion Gene Detection Test

Unique
The only commercial qPCR kit for EML4-ALK and KIF5B-ALK fusion variants

Specific & sensitive
No cross-reactivity with wild-type RNA & 50 fusion templates detected per reaction

Comprehensive
Covers 95% EML4-ALK and 90% KIF5B-ALK variants in COSMIC database

Rapid
Total turnaround time approximately 1.5 hours

Easy to operate
One-tube multiplex qPCR reactions and only 50 ng RNA as assay input

Great VERSATILITY
Assay validated in widely used real-time PCR machines
Product Specifications for QFusion™ EML4-ALK and KIF5B-ALK Fusion Gene Detection Test
Product Catalog Number
CE/IVD Catalog Number: DC-20-0020E;
Research-Use-Only (RUO) Catalog Number: DC-20-0020R
Intended Use
For in vitro diagnostic use (CE/IVD) or for research use
Sample Type
FFPE tissues from non-small cell lung cancer (minimum 50 ng RNA)
Fusion Variants
EML4-ALK: V1, V2, V3a/b, V5a/b, V6,V7, V8a/b KIF5B-ALK: (K15; A20), (K17; A20), (K24; A20)
Pack Size
20 Samples
Instruments Validated
Roche LightCycler® 96, Roche LightCycler® 480 II, and Bio-Rad CFX384
Detection Channel
FAM; HEX
Turnaround Time
PCR setup: ~0.5 hour; assay run time: ~1 hour
Stability
Stable for 12 Months at -25°C to -15°C