Dimerator™ Adapter Dimer Removal for NGS Library Preparation
Powered by XNA technology, Dimerator™ uses XNA clamps to block adapter-adapter ligation and improves adapter dimer to tagged library ratio, which lead to significantly higher quality sequencing data. This technology has solved the problem of formed dimer when 5’ and 3’ adapters self-ligate without library insert and requires no gel purification to remove dimer. It also prevent the amplification of the smaller dimer side product out-competes amplification of tagged library.
How it works
After ligating a 3´-adenylated DNA adapter to the 3´-end of miRNA in total RNA, an RNA adapter is ligated to the 5´-end of the miRNA. The ligations also produce an unwanted adapter-dimer side product as shown in the figure. The Dimerator™ works by blocking this side product from amplifying by RT-PCR. However, the doubly ligated products are unaffected by the Dimerator™ and amplified to generate sequencing libraries and introduce the barcodes. Two separate reads are required to sequence the insert miRNA and barcode, respectively, on the flow cell surface with the Illumina NGS platforms.