Dimerator™ Adapter Dimer Removal for NGS Library Preparation
Powered by XNA technology, Dimerator™ uses XNA clamps to block adapter-adapter ligation and improves adapter dimer to tagged library ratio, which lead to significantly higher quality sequencing data. This technology has solved the problem of formed dimer when 5’ and 3’ adapters self-ligate without library insert and requires no gel purification to remove dimer. It also prevent the amplification of the smaller dimer side product out-competes amplification of tagged library.
• Increase valuable sequencing reads
• Handle low inputs and small RNA (e.g. miRNA)
• Tag efficiently in samples with low target abundance
• Eliminate need for gel purification
• Full automation possible
|Product Name||Catalog # (RUO)||Catalog # (CE/IVD)
|Dimerator™ NGS Adapter Dimer Removal Kit (500 Reactions)||DC-60-0500R||N/A|
|Dimerator™ NGS Adapter Dimer Removal Kit (1000 Reactions)||DC-60-1000R||N/A|
Note: RUO (Research Use Only) products are not for use in diagnostic procedures.
How it works
After ligating a 3´-adenylated DNA adapter to the 3´-end of miRNA in total RNA, an RNA adapter is ligated to the 5´-end of the miRNA. The ligations also produce an unwanted adapter-dimer side product as shown in the figure. The Dimerator™ works by blocking this side product from amplifying by RT-PCR. However, the doubly ligated products are unaffected by the Dimerator™ and amplified to generate sequencing libraries and introduce the barcodes. Two separate reads are required to sequence the insert miRNA and barcode, respectively, on the flow cell surface with the Illumina NGS platforms.