Select Page

Customized XNA Service

For Mutation Detection and Screening

Our proprietary XNA technology can enrich mutation sequences while blocking wildtype sequence amplification, thereby increasing mutation detection sensitivity. We have made the customized XNA synthesis and assay development service available for research use only (RUO) applications such as real-time quantitative PCR (qPCR), Sanger sequencing, and CRISPR-based mutant screening.

XNA Application - Increase Assay Sensitivity

XNA technology can be combined with the commonly available detection techniques such as Sanger sequencing, qPCR, and NGS to increase assay sensitivity significantly.

XNA Application - CRISPR

XNA can also be used to screen mutants generated from the CRISPR technique.

XNA Application - COVID-19 Detection

XNA technology has also been applied for COVID-19 variant identification.

GET A QUOTE NOW

Application: CRISPR Mutant Screening Assay

CRISPR is routinely used as a gene-editing tool for mutant creation. However, mutant screening can be a tedious task if the transfection rate of a specific cell line is low.

Our XNAs can be used for both pool screening and individual clone screening for the presence of mutations. While ordering gRNAs, customers can order our XNAs and screening assay at the same time. After transfection is completed, the XNA screening assay is optimized and ready to screen for mutants.

Compared to the traditional assays for mutant screening, the XNA screening assay is more sensitive (point mutations and indels, including small ones), and high throughput screening is also easy to perform using the SYBR Green qPCR assay.

qPCR Assays

For qPCR assays, adding an optimized concentration of XNA to the standard qPCR reactions can block the wildtype sequence template amplification but allows the mutation sequence to be amplified in the qPCR reaction.

The XNA-based mutation enrichment allows low-frequency somatic mutations to be detected in cfDNA or tissue biopsy. The mutation detection sensitivity can reach 0.1% or below, a 10-fold better sensitivity than the traditional qPCR assay without the XNA.

Visit our existing products that use a combination of XNA and qPCR for mutation detection by clicking the button below.

Sanger Sequencing

For Sanger sequencing, the sensitivity is normally at 15 to 20% variant allele frequency. Adding XNA to the template preparation PCR reaction, one can enrich the mutation templates and the mutation can be easily detected by Sanger sequencing.

For instance, the BRAF V600 XNA improves the sensitivity of sanger sequencing downstream to 0.04%, more than 100-fold better than the standard Sanger sequencing. The enhanced sensitivity allows Sanger sequencing to be used for low-frequency mutation detection in cfDNA or tissue biopsy, meeting the needs for companion diagnostics for specific targets.

Visit our existing products that combine XNA with Sanger sequencing for mutation detection by clicking the button below. 

XNA customized service

Package 1: XNA Only

We will synthesize the XNA and design the primers and other necessary components for the assay. Ordering the designed primers, TaqMan probe, or positive control from other vendors using our design information is not included in this package. Ordering master mix or SYBR Green dye from other vendors based on our suggestion (listed in instructions) is not included as well.

The DNA sequence of 100 bp upstream and 100 bp downstream of the mutation site (the cleavage site for CRISPR service) is required。

Delivery window: 5 business days

XNA synthesized

Other necessary components designed

Other necessary components is not included

Optimization is not included

CRISPR

  • XNA synthesized
  • Primers and positive control designed
  • Pack size: 100 reactions (Catalog #: DC-02-1101R)
  • Pack size: 500 reactions (Catalog #: DC-02-1102R)

qPCR

  • XNA synthesized
  • Primers, TaqMan probe, and positive control designed
  • Pack size: 100 reactions (Catalog #: DC-02-1103R)
  • Pack size: 500 reactions (Catalog #: DC-02-1104R)

Sanger Sequencing

  • XNA synthesized
  • Primers and positive control designed
  • Pack size: 100 reactions (Catalog #: DC-02-1105R)
  • Pack size: 500 reactions (Catalog #: DC-02-1106R)

XNA customized service

Package 2: Synthesis Only

We will synthesize the XNA, primers, TaqMan probe, and positive control for you. We will also provide master mix, SYBR Green, or other PCR components if needed in the protocol. Optimizing the assays using the protocols provided in the user manual is not included in this package.

The DNA sequence of 100 bp upstream and 100 bp downstream of the mutation site (the cleavage site for CRISPR service) is required。

Delivery window: 7 business days

XNA synthesized

Other necessary components provided

Optimization is not included

CRISPR

  • XNA, primers and positive control designed and synthesized
  • Master mix and SYBR Green provided
  • Pack size: 100 reactions (Catalog #: DC-02-1201R)
  • Pack size: 500 reactions (Catalog #: DC-02-1202R)

qPCR

  • XNA, primers, TaqMan probe, and positive control designed and synthesized
  • Master mix provided 
  • Pack size: 100 reactions (Catalog #: DC-02-1203R)
  • Pack size: 500 reactions (Catalog #: DC-02-1204R)

Sanger Sequencing

  • XNA, primers and positive control designed and synthesized
  • Master mix provided
  • Pack size: 100 reactions (Catalog #: DC-02-1205R)
  • Pack size: 500 reactions (Catalog #: DC-02-1206R)

XNA customized service

Package 3: Optimized Assay

We will design, synthesize and optimize the PCR/qPCR assay for you to use directly.

The DNA sequence of 100 bp upstream and 100 bp downstream of the mutation site (the cleavage site for CRISPR service) is required.

Delivery window: 5 business days

XNA synthesized

Other necessary components provided

Optimization included

CRISPR

  • All assay components are included, and the assay is optimized and ready to use
  • Pack size: 100 reactions (Catalog #: DC-02-1301R)
  • Pack size: 500 reactions (Catalog #: DC-02-1302R)

qPCR

  • All assay components are included, and the assay is optimized and ready to use
  • Pack size: 100 reactions (Catalog #: DC-02-1303R)
  • Pack size: 500 reactions (Catalog #: DC-02-1304R)

Sanger Sequencing

  • All assay components are included, and the assay is optimized and ready to use
  • Pack size: 100 reactions (Catalog #: DC-02-1305R)
  • Pack size: 500 reactions (Catalog #: DC-02-1306R)

Resources

Need Help?

Call us: +1 (800) 246-8878

Email us: information@diacarta.com

Contact Us!

Ready to Subscribe and Learn?

Register and Learn!

Join our mailing list to receive the latest news and updates from our team.

You have Successfully Subscribed!

X